Enzymatic functionalization of hemicellulose to produce biobased cross-linkers

The overall aim of this project is to enzymatically modify glycans from hemicellulose to produce bio-based cross-linkers for applications in adhesives, hydrogels and other cross-linked materials. Many materials that we consider as bio-based such as wood fibreboards, hydrogels and stabilizers from non-fossil polymers are still dependent on cross-linkers that are of fossil origin and often very harmful chemicals. Fibreboards for furniture, construction and insulation typically contains about 9% adhesive, using a formaldehyde-urea resin. Formaldehyde is classified as a known human carcinogen and its emission from the fibreboards is a major concern.In this project, oxidative enzymes will be used on hemicellulose substrates, such as xyloglucan and glucomannan, to obtain aldehyde and ketone functions on the sugar ring in parallel with a partial degradation to oligosaccharides. The expected result is a collection of non-toxic, bio-based cross-linkers that can potentially replace formaldehyde and similar fossil-based cross-linkers.The project will be performed with an advanced level of structural characterization using NMR spectroscopy and mass spectrometry to be able to draw conclusions about the structural composition of the cross-linkers and the cross-linked materials. The project is a collaboration between Dept of Molecular Sciences and Dept of Forest Bioeconomy and Technology at SLU, Uppsala.